Differentiation Protocol for 3D Retinal Organoids, Immunostaining and Signal Quantitation
نویسندگان
چکیده
منابع مشابه
Immunostaining for cell picking and real-time mRNA quantitation.
Microdissection techniques allow a cell-type or even cell-specific mRNA analysis within complex tissues. Furthermore, valid mRNA quantitation can be performed by real-time reverse transcriptase-polymerase chain reaction from a few isolated cells obtained from cryosections. For a more precise access to many cell types, this technique has to be complemented by a cell-type-specific immunostaining....
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Purpose We aimed to establish purification and culture systems for retinal ganglion cells (RGCs) differentiated from mouse and human pluripotent stem cells (PSC) for in vitro and regenerative medicine studies. Methods We used a two-step immunopanning method to purify RGCs from mouse and human PSC-derived three-dimensional (3D) retinal organoids. To assess the method, we purified RGCs from 3D ...
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ژورنال
عنوان ژورنال: Current Protocols in Stem Cell Biology
سال: 2020
ISSN: 1941-7322,1938-8969
DOI: 10.1002/cpsc.120